Author(s): Dr. Manav Patel, Dr. Rekha Parmar, Dr. Aparna Bagul3, Dr Hitaba Gohil
Authors Affiliations:
1PG Scholar Department of PG & Ph.D. Studies in Dravya guna, Parul Institute of Ayurveda, Parul University, Vadodara, Gujarat, ORCID ID – 0009-0008-5291-6463 .
2Professor Department of PG & Ph.D. Studies in Dravya guna, Parul Institute of Ayurveda, Parul University, Vadodara, Gujarat.
3Professor and H.O.D Department of Swasthavritta evam Yoga, Parul Institute of Ayurveda, Parul University, Vadodara, Gujarat.
4In charge Quality Control Laboratory, Parul Institute of Ayurveda, Parul University, Vadodara, Gujarat.
DOIs:10.2015/IJIRMF/202604031 | Paper ID: IJIRMF202604031
Abstract: Mango seed kernels are often overlooked as agricultural residues, yet they hold considerable promise for nutraceutical development and Ayurvedic formulations. In the present investigation, the phytochemical composition of both dry and wet kernel extracts was qualitatively assessed through High‑Performance Thin Layer Chromatography (HPTLC). The extracts were spotted on silica gel 60 F254 plates and resolved using a mobile phase of ethyl acetate, glacial acetic acid, formic acid, and water (7:1:1:1.5, v/v/v/v). Visualization was carried out under UV detection at 254 nm (absorbance) and 366 nm (fluorescence). The dry preparation revealed four distinct chromatographic peaks, with a prominent band at Rf 0.786 accounting for 35.84% of the total peak area. In contrast, the wet extract displayed three major peaks, with the same Rf 0.786 band contributing 46.13%. These chromatographic fingerprints suggest the presence of polyphenolic constituents such as mangiferin and quercetin, with notable differences in band intensity between the two extraction modes. Comparative evaluation underscores how extraction conditions influence phytochemical distribution, emphasizing the role of processing in shaping bioactive profiles. Collectively, the findings reinforce the potential of mango seed kernels as sources of antioxidant and antimicrobial agents, resonating with Ayurvedic perspectives on plant‑derived therapeutics. Future work should focus on quantitative estimation and bioavailability studies to substantiate their nutraceutical applications.
Key Words: Mango seed kernel, Qualitative phytochemicals, HPTLC profiling, Polyphenolic compounds, Dry vs wet extracts.
Dr. Manav Patel, Dr. Rekha Parmar, Dr. Aparna Bagul, Dr Hitaba Gohil (2026); Comparative Qualitative Phytochemical Analysis of Dry and Wet Mango Seed Kernel Extracts Using HPTLC, International Journal for Innovative Research in Multidisciplinary Field, ISSN(O): 2455-0620, Vol-12, Issue-4, Available on – https://www.ijirmf.com/
