15, April 2025

Recent Trends in Identification and Characterization of Bacteria causing Waterborne Diseases using Conventional Techniques

VOLUME-11; ISSUE-4; APR-2025

Author(s): Meenal Sahu, Anukriti Saran, Dipjyoti Chakraborty, Swapnil Sharma

Authors Affiliations:

1 Department of Pharmacy, Banasthali Vidyapith, Banasthali, Rajasthan, India

2 Department of Bioscience & Biotechnology, Banasthali Vidyapith, Banasthali, Rajasthan, India

DOIs:10.2015/IJIRMF/2025004008     |     Paper ID: IJIRMF202504008


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This study aimed to detect and isolate bacteria from water samples collected from various sources. Standard bacterial isolation techniques, including serial dilution and streaking methods, were employed. The bacterial species were identified based on colony morphology, biochemical tests, and molecular characterization using the 16S rRNA gene. Bacteriological analysis revealed the presence of Escherichia spp. in potable water samples. The occurrence of Escherichia spp. highlights the necessity for regular sanitation practices, effective water treatment processes, and the management of water resources. This study recommends frequent bacteriological testing of drinking water sources to ensure water quality and safeguard public health.

 
Keywords: Escherichia spp.; Molecular characterization; Biochemical tests; Waterborne diseases; Antibiotics; Serial dilution

Meenal Sahu, Anukriti Saran, Dipjyoti Chakraborty, Swapnil Sharma(2025); Recent Trends in Identification and Characterization of Bacteria causing Waterborne Diseases using Conventional Techniques, International Journal for Innovative Research in Multidisciplinary Field, ISSN(O): 2455-0620, Vol-11, Issue-4, Pp.56-60.          Available on –   https://www.ijirmf.com/

  1. Sharma, S., Sachdeva, P., & Virdi, J. S. (2003). Emerging water-borne pathogens. Applied Microbiology and Biotechnology, 61, 424-428.
  2. Agingu, J. B. (2020). Levels and differentials of occurence of water borne diseases at Moi University, Kenya.
  3. Bidhuri, S., Taqi, M., & Khan, M. M. A. (2018). Water-borne disease: Link between human health and water use in the Mithepur and Jaitpur area of the NCT of Delhi. Journal of Public Health, 26, 119-126.
  4. Romaniuk, S. N., & Marton, P. (Eds.). (2023). The Palgrave Encyclopedia of Global Security Studies. Springer International Publishing.
  5. WHO/UNICEF Joint Water Supply, & Sanitation Monitoring Programme. (2015). Progress on sanitation and drinking water: 2015 update and MDG assessment. World Health Organization.
  6. Szálkai, K. (2023). Water-borne diseases. In The Palgrave Encyclopedia of Global Security Studies (pp. 1540-1546). Cham: Springer International Publishing.
  7. Şahin, S., Sivri, N., Akpinar, I., Çinçin, Z. B., & Sönmez, V. Z. (2021). A comprehensive bibliometric overview: antibiotic resistance and Escherichia coli in natural water. Environmental Science and Pollution Research, 28(25), 32256-32263.
  8. Garrity G.M. (2005). The proteobacteria. 2nd ed., In: Brenner DJ, Krieg NR, Staley JT. Bergey’s manual of Systematic Bacteriology, 2: 325-340.
  9. Kavitha, R., Dhamodharan, N., & Dhivya, C. (2017). Screening, isolation, and antibacterial activity of antibiotic producing bacteria obtained from saprophytic soil samples. Asian Journal of Pharmaceutical and Clinical Research, 10(3), 92-96.
  10. Humayun, E., Bibi, A., Rehman, A. U., Ahmad, S., & Shujaat, N. (2015). Isolation and identification of coliform bacteria from drinking water sources of Hazara Division, Pakistan. IOSR Journal of Pharmacy, 5(4), 36-40.
  11. Abiola, C., & Oyetayo, V. O. (2016). Research Article Isolation and Biochemical Characterization of Microorganisms Associated with the Fermentation of Kersting’s Groundnut (Macrotyloma geocarpum). Journal of Microbiology, 11, 47-55.
  12. Masi, C., Gemechu, G., & Tafesse, M. (2021). Isolation, screening, characterization, and identification of alkaline protease-producing bacteria from leather industry effluent. Annals of Microbiology, 71, 1-11.
  13. Pandey, S., & Chakraborty, D. (2019). Isolation and characterization of Rhizobium sp. from a collar rot tolerant groundnut (Arachis hypogaea L.) variety. Plant Science Today, 6(sp1), 631-634.
  14. Buxton, R. (2011). Nitrate and nitrite reduction test protocols. American Society for Microbiology, 1-20.
  15. Bauer, A. W., Kirby, W. M. M., Sherris, J. C., & Turck, M. (1966). Antibiotic susceptibility testing by a standardized single disk method. American journal of clinical pathology, 45(4_ts), 493-496.

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