20, May 2025

Typing of Candida albicans in UTI and Oral Swabs Using Gel Electrophoresis and Antifungal Assays

VOLUME-11; ISSUE-5; MAY-2025

Author(s): Prabhudatta Behera, Jharna Maiti, Rupesh Thakur, Kiranbala Das,

Authors Affiliations:

1Department of Biochemistry, Kalinga University, Naya Raipur (C.G.) 492101

2 Research Associate, Heredity Biosciences, Bhubaneswar, India

DOIs:10.2015/IJIRMF/202505005     |     Paper ID: IJIRMF202505005


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Abstract :  This abstract is a summary of a study that examined Candida albicans strains isolated from Urinary Tract Infections (UTIs) and Oral Candidiasis to determine their molecular epidemiology, phenotypic features, and antifungal susceptibility. Candida albicans is an important opportunistic fungal pathogen, and the frequency of infections, such as UTIs and oral candidiasis, and antifungal resistance presents a clinical problem. This research utilized Molecular Typing based on Random Amplified Polymorphic DNA-PCR (RAPD-PCR) and Gel Electrophoresis for differential strain and Genotype identification. Phenotypic traits, such as Antifungal Susceptibility Testing in response to ordinary antifungals such as Fluconazole, were determined as well. The findings exhibited high genetic divergence among C. albicans from various body localizations, whereby different genotypes were correlated with UTIs as well as infections of the mouth. A significant observation was the identification of Fluconazole Resistance in a portion of isolates, which indicates possible treatment implications. Specific genotypes and resistance patterns were correlated, indicating dissemination of resistant clones. The present work adds to the knowledge of the Epidemiology and strain dynamics of C. albicans infections and emphasizes the significance of molecular typing and susceptibility testing for effective control and management of candidiasis.

     
Keywords: Candida albicans; Urinary Tract Infection (UTI); Oral Candidiasis; Molecular Typing; RAPD-PCR; Gel Electrophoresis; Antifungal Susceptibility Testing; Fluconazole Resistance; Strain Differentiation; Genotype; Phenotype; Epidemiology.

Prabhudatta Behera, Jharna Maiti, Rupesh Thakur(2025); Typing of Candida albicans in UTI and Oral Swabs Using Gel Electrophoresis and Antifungal Assays,  International Journal for Innovative Research in Multidisciplinary Field, ISSN(O): 2455-0620, Vol-11, Issue-5, Pp.26-36.          Available on –   https://www.ijirmf.com/

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