Abstract: The phytochemical investigation of the whole plant of Zornia gibbosa revealed the presence of secondary metabolites. The antimicrobial efficiency and minimum inhibition concentration values were determined for these phytochemical constituents as crude extracts using the agar well diffusion and two-fold serial dilution methods, respectively. The results indicated that Klebsiella pneumoniae was the most susceptible bacterium with high inhibition zones for the methanol and chloroform extracts of 30 mm and 24 mm, respectively. The minimum inhibitory concentration values indicated that extracts possess good phytochemicals with significant minimum inhibitory concentration value against Klebsiella pneumoniae and Escherichia coli at 15.6 µg/ml concentration. Gram-negative bacteria were more susceptible than the Gram-positive. The present study suggests that Zornia gibbosa whole plant possess potent antimicrobial efficiency and can be a good source for the development of new antibiotics.